Journal
BIO-PROTOCOL
Volume 11, Issue 7, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3969
Keywords
Atomic force microscopy; Ginger lipid-derived nanoparticles; Colon-targeted drug delivery; 6-shogaol; Metabolites of 6-shogaol
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Funding
- National Institute of Diabetes and Digestive and Kidney Diseases [RO1-DK-116306, RO1-DK-107739]
- Department of Veterans Affairs [BX002526, BX004476]
- Crohn's and Colitis Foundation of America
- Crohn's and Colitis Foundation of America [689659]
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Our study demonstrated that ginger-derived nanoparticles could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. We successfully constructed ginger lipid-derived nanoparticles as a carrier to deliver drug candidates to the colon, providing a detailed protocol for this process. Using atomic force microscopy, we visualized the nanostructure of the drug-loaded nanoparticles.
We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols). To construct a nanocarrier that is more clearly defined than GDNP-2, we isolated lipids from GDNP-2 and demonstrated that they could self-assemble into ginger lipid-derived nanoparticles (GLDNP) in an aqueous solution. GLDNP can be used as a nanocarrier to deliver drug candidates such as 6-shogaol or its metabolites (M2 and M13) to the colon. To characterize the nanostructure of GLDNP, our lab extensively used atomic force microscopy (AFM) technique as a tool for visualizing the morphology of the drug-loaded GLDNP. Herein, we provide a detailed protocol for demonstrating such a process.
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