Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 110, Issue -, Pages 340-350Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2017.10.042
Keywords
Oxidative stress; Copper; Quercetin; Fenton reaction; EPR spectroscopy; DNA damage
Categories
Funding
- Scientific Grant Agency (VEGA) [1/0686/17, 1/0041/15, 1/0594/16]
- Research and Development Support Agency [APVV-15-079]
- Grant Agency of Constantine Philosopher University in Nitra (UGA) [VII/6/2014]
- European Commission [26220220180]
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The radical scavenging and metal chelating properties of flavonoids indicate that they may play a protective role in diseases with perturbed metal homeostasis such as Alzheimer's disease. In this work we investigated the effect of the coordination of quercetin to copper(II) in view of the formation of ROS in Cu-catalyzed Fenton reaction. ABTS and DPPH assays confirmed that the copper(II)-quercetin complex exhibits a stronger radical scavenging activity than does quercetin alone. EPR spin trapping experiments have shown that chelation of quercetin to copper significantly suppressed the formation of hydroxyl radicals in the Cu(II)-Fenton reaction. DNA damage experiments revealed a protective effect for quercetin, but only at higher stoichiometric ratios of quercetin relative to copper. DNA protective effect of quercetin against ROS attack was described by two mechanisms. The first mechanism lies in suppressed formation of ROS due to the decreased catalytic action of copper in the Fenton reaction, as a consequence of its chelation and direct scavenging of ROS by free quercetin. Since the Cu-quercetin complex intercalates into DNA, the second mechanism was attributed to a suppressed intercalating ability of the Cu-quercetin complex due to the mildly intercalating free quercetin into DNA, thus creating a protective wall against stronger intercalators.
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