Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 105, Issue -, Pages 241-255Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2017.04.024
Keywords
Actinomyces; Bioactive secondary metabolites; Aflatoxin B1; Oxidative stress; cytotoxicity; Liver
Categories
Funding
- National Research Centre, Dokki, Cairo, Egypt project [11090341, 10070208]
Ask authors/readers for more resources
This study aimed to determine the bioactive compounds of actinomyces (ACT) isolated from the Egyptian environment (D-EGY) and to evaluate their protective activity against AFB(1) in female Sprague-Dawley rats. Six groups of animals were treated orally for 3 weeks included: C, the control group, Tl, AFB(1)-treated group (80 mu g/kg b.w), T2 and T3, the groups received ACT extract at low (25 mg/kg b.w) or high (50 mg/kg b.w) doses, T4 and T5, the groups received AFB(1) plus the low or high dose of ACT extract. Blood, bone marrow and tissue samples were collected for different analyses and histological examination. The results revealed the identification of 40 components, representing 99.98%. Treatment with AFB(1) disturbs liver function parameters, oxidative stress markers, antioxidant gene expressions, DNA fragmentation and induced severe histological changes. ACT extract at the low or high doses did not induce significant changes in all the tested parameters or histological picture of the liver. Moreover, ACT extract succeeded to induce a significant protection against the toxicity of AFB(1). It could be concluded that the bioactive compounds in ACT are promise candidate for the development of food additive or drugs for the protection and treatment of liver disorders in the endemic area. (C) 2017 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available