Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 106, Issue -, Pages 165-174Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2017.05.037
Keywords
Oxidative stress; Mitochondrial reactive oxygen species; Polyphenols; Neoplastic transformation; Benzo [a]pyrene; Carcinogenesis
Categories
Funding
- Saskatchewan Health Research Foundation (SHRF)
- Natural Sciences and Engineering Research Council (NSERC)
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While dietary polyphenols are widely recognized for cancer-preventing characteristics, the relative effectiveness and mechanisms of action of different polyphenols is not clear. In the present study, we investigated the protective effects of six different polyphenols against benzo[a]pyrene (B[a]P)-induced oxidative stress and neoplastic transformation in the Bhas 42 cell carcinogenesis assay. All of the polyphenols completely prevented the increased intracellular ROS generation by B[a]P at 12 h, and most inhibited after 3 days. B[a]P increased mitochondrial superoxide generation at 12 h, which was inhibited by the anthocyanins and berberine. B[a]P increased expression of genes related to oxidative stress and inflammation (Nrf2, UCP2, and TNF-alpha) after 24 h. Polyphenols strongly inhibited the increase in TNF-a and also several polyphenols inhibited the increase in UCP2. At 21 days after 72 h treatment, B[a]P produced a large increase in the number of neoplastic colonies. This transformation was inhibited by most polyphenols, and strongly by resveratrol. In summary, all tested polyphenols were able to inhibit B[a]P-induced increases in markers of oxidative stress and inflammation, and to inhibit cellular transformation, with resveratrol being notable for the strongest preventive effect on cell transformation. The results support a role for dietary polyphenols in protecting against B[a]P-induced carcinogenesis. (C) 2017 Elsevier Ltd. All rights reserved.
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