Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 28, Issue 4, Pages 625-638Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1309358
Keywords
beta-agonist; ic-ELISA; immunochromatographic strip; urine
Funding
- National Key RD Program [2016YFF0202300]
- National Natural Science Foundation of China [21673104]
- Natural Science Foundation of Jiangsu Province, MOF [BE2014672, CMB21S1614]
- TaiShan Industry Leading Talent Special Funds
- Innovative Project of Postgraduate Education in Jiangsu Province [SJLX150542]
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A sensitive monoclonal antibody, 3G10, derived from a mouse immunized with an immunogen of salbutamol hapten conjugated to keyhole limpet hemocyanin was developed against beta-agonists, with a view to establishing a rapid gold nanoparticle immunochromatographic strip. The antibody displayed a 50% inhibitory concentration (IC50) of 1.04ng/mL for salbutamol and simultaneously detected nine other beta-agonists (clenbuterol, cimatero, brombuterol, mabuterol, terbutaline, clenpenterol, carbuterol, mapenterol, pirbuterol) with uniform cross-reactivity. The immunochromatographic strip had cut-off values of 5ng/mL for salbutamol and clenbuterol, 20ng/mL for cimaterol, brombuterol and mabuterol, and 50ng/mL for terbutaline, clenpenterol, carbuterol, mapenterol and pirbuterol. The collective advantages of the newly developed strip, such as high affinity, sensitivity and rapidity, support its utility in detecting beta-agonists from actual biological samples, such as urine.
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