Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 28, Issue 3, Pages 427-438Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1293015
Keywords
Aldicarb; immunochromatographic assay; monoclonal antibody; pesticide; test strip
Funding
- Natural Science Foundation of China [21471068]
- Key Programs from MOST [2016YFD0401101, 2016YFF0202300]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [201513006, BE2016307, BK20140003, CMB21S1614]
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A rapid, simple, and sensitive immunochromatographic strip test has been developed to detect aldicarb (ALD) in 0.01 M phosphate buffer solution (PBS) (pH 7.4) and cucumber samples. The test was developed with a sensitive monoclonal antibody specific for ALD, generated by immunizing BALB/c mice with a well-characterized ALD - keyhole limpet hemocyanin conjugate, produced in our laboratory. Under the optimized condition, the cut-off limits of test strip for ALD were found to be 25ng/mL in 0.01 M PBS (pH 7.4) and 100ng/mL in cucumber samples, and the highest standard relative deviation was 9.05%. Both results were obtained within 5 min. The data indicate that the method is sensitive, rapid, and specific, so this immunochromatographic test strip has utility for the rapid high-throughput screening of the pesticide, especially in food samples.
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