Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 28, Issue 3, Pages 403-413Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1293013
Keywords
Furazolidone; 3-amino-2-oxazolidinone; immunochromatography test strip; colloidal gold nanoparticles
Funding
- Scientific and Technological Research Project of Chongqing [cstc2016shmszx80069]
- China Scholarship Council [CSC] [201608505051]
- National Natural Science Foundation of China [31671939]
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3-amino-2-oxazolidinone (AOZ) is the metabolite of furazolidone, which has been banned as a veterinary drug due to the potential harmful effects on human health. In this study, a novel immunochromatography test strip (ICTS) based on colloidal gold-McAb probe for detection of AOZ within tissue samples was developed. With this method, the visual cut-off levels for the test strip was achieved at 10g/L. Using a test strip reader, the 50% inhibition was 1.3g/L. The limit of detection and limit of quantification in four kinds of animal tissues were 0.15g/kg and 0.31g/kg, respectively. When used to analyze fortified samples of animal tissue, acceptable recovery rates of 76.3-98.4% were obtained. The fortified samples were detected by ICTS and confirmed by LC-MS/MS. The results of the two methods were in good agreement. The proposed ICTS was demonstrated to be a rapid and simple analytical method for detecting AOZ in animal tissue.
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