Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 28, Issue 6, Pages 1105-1115Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1328662
Keywords
Casein; dual-labelled immunoassay; nanoprobe; milk allergen
Funding
- State Key Laboratory of Dairy Biotechnology Industry (Bright Dairy Limited by Share Ltd) [SKLDB2013-04]
- Key Project of Shanghai Agriculture Prosperity through Science and Technology [2016 (4-4)]
- Capacity Building of Local Colleges and Universities in Shanghai [13430502900]
- China Postdoctoral Science Foundation [2016M591698]
- Agricultural Achievement Transformation Fund [143919N0500]
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A dual-labelled immunoassay using goldmag nanoparticles (GMNPs) and CdTe quantum dots (QDs) was applied for the quantification of casein in milk. In this method, anti-casein monoclonal antibody bound to GMNPs was used as capture probe, and the anti-casein polyclonal antibody, labelled with CdTe QDs, was employed as detection probe. This dual-labelled immunoassay was optimized and applied in the testing of casein content of three brands of commercial milks. The results showed a good linear tendency between casein concentrations and fluorescent intensities in the range of 4.617-289.9 ng/mL, and the half inhibition concentration (IC50) was 36.59 ng/mL. Besides, the recovery rates of this developed immunoassay were in accordance with those in traditional enzyme-linked immunosorbent assay for rapid detection of casein content in commercial milks. It suggested that this dual-labelled immunoassay was reliable, and could provide important theoretical value and practical significance in the identification and quantification of milk allergens.
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