LED spectral quality and NaCl salinity interact to affect growth, photosynthesis and phytochemical production of Mesembryanthemum crystallinum

The edible halophyte Mesembryanthemum crystallinum L. was grown at different NaCl salinities under different combined red and blue light-emitting diode (LED) light treatments. High salinity (500 mM NaCl) decreased biomass, leaf growth, and leaf water content. Interactions between LED ratio and salinity were detected for shoot biomass and leaf growth. All plants had F-v/F-m ratios close to 0.8 in dark-adapted leaves, suggesting that they were all healthy with similar maximal efficiency of PSII photochemistry. However, measured under the actinic light near or above the growth light, the electron transport rate (ETR) and photochemical quenching (qP) of M. crystallinum grown at 100 and 250 mM NaCl were higher than at 500 mM NaCl. Grown under red/blue LED ratios of 0.9, M. crystallinum had higher ETR and qP across all salinities indicating higher light energy utilisation. Crassulacean acid metabolism (CAM) was induced in M. crystallinum grown at 500 mM NaCl. CAM-induced leaves had much higher non-photochemical quenching (NPQ), suggesting that NPQ can be used to estimate CAM induction. M. crystallinum grown at 250 and 500 mM NaCl had higher total chlorophyll and carotenoids contents than at 100 mM NaCl. Proline, total soluble sugar, ascorbic acid, and total phenolic compounds were higher in plants at 250 and 500 mM NaCl compared with those at 100 mM NaCl. An interaction between LED ratio and salinity was detected for proline content. Findings of this study suggest that both salinity and light quality affect productivity, photosynthetic light use efficiency, and proline accumulation of M. crystallinum.

Automated summary

The study revealed that both salinity and light quality have effects on the growth, photosynthetic efficiency, and proline accumulation of M. crystallinum. High salinity induced Crassulacean acid metabolism (CAM) in the plant, leading to higher non-photochemical quenching (NPQ) in the leaves.