Journal
CADERNOS DE SAUDE PUBLICA
Volume 37, Issue 3, Pages -Publisher
CADERNOS SAUDE PUBLICA
DOI: 10.1590/0102-311X00041320
Keywords
Enzyme-Linked Immunosorbent Assay; Visceral Leishmaniasis; Dogs; Diagnosis
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Funding
- Ezequiel Dias Foundation (Funed)
- Brazilian National Research Council (CNPq)
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The ELISA-Vetlisa/BIOCLIN prototype showed high sensitivity and specificity in diagnosing canine visceral leishmaniasis, with consistent performance across dogs with different clinical statuses. The agreement between repeated test results was almost perfect, indicating promising potential for use in Brazil.
Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vettisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.
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