Protein-based lateral flow assays for COVID-19 detection

To combat the enduring and dangerous spread of COVID-19, many innovations to rapid diagnostics have been developed based on proteinprotein interactions of the SARS-CoV-2 spike and nucleocapsid proteins to increase testing accessibility. These antigen tests have most prominently been developed using the lateral flow assay (LFA) test platform which has the benefit of administration at point-of-care, delivering quick results, lower cost, and does not require skilled personnel. However, they have gained criticism for an inferior sensitivity. In the last year, much attention has been given to creating a rapid LFA test for detection of COVID-19 antigens that can address its high limit of detection while retaining the advantages of rapid antibodyantigen interaction. In this review, a summary of these proteinprotein interactions as well as the challenges, benefits, and recent improvements to protein based LFA for detection of COVID-19 are discussed.

Automated summary

In order to combat the spread of COVID-19, rapid diagnostic innovations based on protein-protein interactions have been developed, particularly antigen tests using the LFA test platform. Despite the convenience and lower cost of these tests, they have faced criticism for lower sensitivity, prompting recent efforts to create rapid LFA tests with higher limit of detection.