Journal
FITOTERAPIA
Volume 120, Issue -, Pages 146-157Publisher
ELSEVIER
DOI: 10.1016/j.fitote.2017.06.010
Keywords
Grape stems' polyphenols; Preparative-HPLC; Vitamin E; Vitamin C; Inflammation; Oxidative stress
Categories
Funding
- FCT Portuguese Foundation for Science and Technology [UID/AGR/04033/2013, POCI-01-0145-FEDER-006958]
- I & D project Interact - Integrative Research in Environment, Agro-Chains and Technology [NORTE-01-0145-FEDER-000017]
- European Regional Development Fund (FEDER) through NORTE
- Agency for Science and Technology of the Region de Murcia (Spain) [20024/SF/16]
- Fundacao para a Ciencia e Tecnologia of Portugal [SFRH/BD/78013/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/78013/2011] Funding Source: FCT
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The antioxidant potential of grape (Vitis vinifera L.) stems has been reported in the last decade although no identification of the individual compounds responsible for such action has been done. In this work, polyphenolic extract of grape stems was processed resorting to semi-preparative HPLC, allowing to obtain 5 purified poly phenols (caftaric acid, malvidin-3-O-glucoside, quercetin-3-O-glucuronide, mailvidin-3-O-(6-O-caffeoyl)-glucoside, and Sigma-viniferin), which were fully characterized by HPLC-PDA-ESI-MSn. Isolated compounds were featured on their radical scavenging capacity (DPPH and ABTS), cell viability, anti-inflammatory activity, and capacity to modulate the level of reactive oxygen species, glutathione, lipid peroxidation, and overall oxidative stress in a biological model (human keratinocytes) in vitro, under basal and oxidative conditions. The results obtained noticed the combinations malvidin-3-O-glucoside + Vitamin E and quercetin-3-O-glucuronide + vitamin C as the most effective, allowing to improve the capacity of complete extracts or individual compounds, and being candidates to be used in the development of new functional products.
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