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Advances in the Integration of Nucleic Acid Nanotechnology into CRISPR-Cas System

Journal

JOURNAL OF ANALYSIS AND TESTING
Volume 5, Issue 2, Pages 130-141

Publisher

SPRINGER SINGAPORE PTE LTD
DOI: 10.1007/s41664-021-00180-1

Keywords

CRISPR-Cas; Nucleic acid nanotechnology; Gene editing; Gene regulation; CRISPR-based biosensing

Funding

  1. National Natural Science Foundation of China [22034002, 21725503]

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By integrating nucleic acid nanotechnology into the CRISPR-Cas system, advancements have been made in delivering Cas proteins, creating conditional CRISPR-Cas systems based on dynamic RNA nanotechnology, and coupling CRISPR-Cas with DNA origami. These integrations have inspired new possibilities and potential development prospects for the CRISPR-Cas system.
The microbial adaptive immune systems composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), have been repurposed as revolutionary tool kits in many fields, including gene editing, transcriptional regulation, bioimaging and biosensing. Owing to the unprecedented programmability of base paring in nucleic acids, the progress in nucleic acid nanotechnology has brought new inspiration to CRISPR-Cas system. In this mini review, we summarized the research progress of the integration of nucleic acid nanotechnology into CRISPR-Cas system, including delivery of Cas proteins by DNA nanovehicles, conditional CRISPR-Cas system based on dynamic RNA nanotechnology, coupling of CRISPR-Cas and DNA origami. We also discussed the development prospects of the potential combinations of CRISPR-Cas system and nucleic acid nanotechnology.

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