Journal
FISH PATHOLOGY
Volume 52, Issue 4, Pages 210-213Publisher
JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR
DOI: 10.3147/jsfp.52.210
Keywords
Megalocytivirus; red sea bream iridovirus; immunization; immersion; Oplegnathus fasciatus
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Republic of Korea [NRF-2015R1D1A3A01016558]
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Rock bream Oplegnathus fasciatus were immersed in cultured RSIV suspension at 17 degrees C for 30 min, and reared for 80 days without controlling temperature. Fish rearing temperature naturally increased at approximately 0.1 degrees C/day from 17 degrees C. Mortalities of fish without and with RSIV at 10(5.8), 10(3.8) and 10(1.8) TCID50/mL were 5.8%, 30.8%, 22.5% and 6.7%, respectively. Surviving fish were reared at 26 degrees C, and intramuscularly injected with RSIV at 10(1.8) or 10(08) TCID50/fish. Survivors from RSIV-immersion at >= 10(3.8) TCID50/mL were strongly protected against RSIV challenge whereas those at <= 10(1.8) TCID50/mL died, suggesting that fish immunization with live RSIV at low temperature could be conducted by immersion route.
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