Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 66, Issue -, Pages 300-306Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.05.036
Keywords
Nanoparticles; Immunotoxicity; Blood clam; Gene expression; Pattern Recognition Receptors
Funding
- National Natural Science Foundation of China [31672634]
- Open Fund of Key Laboratory for Ecological and Environment in Costal Areas, SOA [201603]
- Open Fund of Key Laboratory of Exploration & Preservation of Costal Bioresources of Zhejiang [J2015002, J2016001]
Ask authors/readers for more resources
The increasing production and extensive application of nanoparticles (NPs) inevitably leads to increased release of NPs into the marine environment and therefore poses a potential threat to marine organisms, especially the sessile benthic bivalves. However, the impacts of NPs on the immunity of commercial and ecological important bivalve species, Tegillarca granosa, still remain unknown to date. In addition, the molecular mechanism of the immunotoxicity of NPs still remains unclear in marine invertebrates. Therefore, the immunotoxicity of nTiO(2) exposure to T. granosa at environmental realistic concentrations was investigated in the present study. Results obtained showed that the total number, phagocytic activity, and red granulocytes ratio of the haemocytes were significantly reduced after 30 days nTiO(2) exposures at the concentrations of 10 and 100 mu g/L. Furthermore, the expressions of genes encoding Pattern Recognition Receptors (PPRs) and downstream immune-related molecules were significantly down regulated by nTiO(2) exposures, indicating a reduced sensitivity to pathogen challenges. In conclusion, evident immunotoxicity of nTiO(2) to T. granosa at environmental realistic concentrations was detected by the present study. In addition, the gene expression analysis suggests that the PRRs (both TLRs and RIG1 investigated) may be the molecules for NPs recognition in marine invertebrates. (C) 2017 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available