Biofabricated silver nanoparticles exhibit broad-spectrum antibiofilm and antiquorum sensing activity against Gram-negative bacteria

The emergence and spread of antimicrobial resistance (AMR) among bacterial pathogens have created a global threat to human health and the environment. Targeting the quorum sensing (QS) linked virulent traits of bacteria is considered to be a novel approach for addressing the problem of AMR. In this study, green synthesized silver nanoparticles (AgNPs-MK) were evaluated for the inhibition of the formation of biofilms and quorum sensing controlled virulence factors against three Gram negative bacteria. Remarkable inhibition (>80%) of QS-mediated violacein production was recorded in C. violaceum 12472. Up to 90% inhibition of the QS-mediated virulent traits of S. marcescens MTCC 97 was observed. The virulence factors of P. aeruginosa PAO1 also decreased in a dose dependent manner in the presence of AgNPs-MK. Moreover, the development of biofilms of C. violaceum 12472, S. marcescens MTCC 97, and P. aeruginosa PAO1 was reduced by 87.39, 81.54, and 71.34%, respectively. Biofilms on glass surfaces were remarkably reduced, with less aggregation of bacterial cells and the reduced formation of extra polymeric substances. The findings clearly show the efficacy of AgNPs-MK against the development of biofilms and the QS mediated virulent traits of Gram negative bacterial pathogens. AgNPs-MK may be further exploited for the development of alternative antimicrobial agents after careful scrutiny in animal models for the management of bacterial infections, especially for topical applications.

Automated summary

The study showed that green synthesized silver nanoparticles (AgNPs-MK) demonstrated significant inhibitory effects on biofilm development and quorum sensing controlled virulence factors of Gram negative bacteria, indicating their potential in addressing the issue of antimicrobial resistance.