Journal
BIORESOURCES AND BIOPROCESSING
Volume 8, Issue 1, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1186/s40643-021-00398-y
Keywords
Impairing photorespiration; Photosynthetic conversion of CO2; Isoprene production; Glycolate dehydrogenase; Cyanobacteria
Categories
Funding
- National Natural Science Foundation of China [31670048, 31470231]
- Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-KJGG-007]
- Key Research Program of the Chinese Academy of Sciences [ZDRW-ZS-2016-3]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA07040405]
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The study demonstrates that by impairing photorespiration in cyanobacteria, the saved energy can be redirected to increase the production of chemicals from CO2.
Photorespiration consumes fixed carbon and energy generated from photosynthesis to recycle glycolate and dissipate excess energy. The aim of this study was to investigate whether we can use the energy that is otherwise consumed by photorespiration to improve the production of chemicals which requires energy input. To this end, we designed and introduced an isoprene synthetic pathway, which requires ATP and NADPH input, into the cyanobacterium Synechocystis sp. 6803. We then deleted the glcD1 and glcD2 genes which encode glycolate dehydrogenase to impair photorespiration in isoprene-producing strain of Synechocystis. Production of isoprene in glcD1/glcD2 disrupted strain doubled, and stoichiometric analysis indicated that the energy saved from the impaired photorespiration was redirected to increase production of isoprene. Thus, we demonstrate we can use the energy consumed by photorespiration of cyanobacteria to increase the energy-dependent production of chemicals from CO2.
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