Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 64, Issue -, Pages 137-145Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.03.003
Keywords
Takifugu obscurus; Cold stress; Apoptosis; Oxidative stress
Funding
- science and technology achievement transformation funds of Ministry of Agriculture [2013GB2E000365]
- Special Funds for Fish Diseases of Guangdong province [2015-352, 2016-302]
- Marine Fisheries Technology and Industry Development Special Projects of Guangdong Province [A201501B14]
- Marine Fisheries Science and Technology Extension Projects of Guangdong Province [B201601-11]
- Science and technology Projects of Guangdong Province [2014B040404057]
- Hainan Applied Technology Research and Development and Demonstration Projects [ZDXM2015025]
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Low temperature is an important environmental factor in aquaculture farming that affects the survival and growth of organisms. In the present study, we investigated the effects of low temperature on biochemical parameters, oxidative stress and apoptosis in pufferfish. In the stress group, water temperature decreased from 25 degrees C to 13 degrees C at a rate of 1 degrees C/1 h. Fish blood and liver were collected to assay biochemical parameters, oxidative stress and expression of genes at 25 degrees C, 21 degrees C, 17 degrees C, 13 degrees C and 13 degrees C for 24 h. The results showed that low temperature could decrease total blood cell count, inhibit cell viability, and subsequently lead to DNA damage. Biochemical parameters such as plasma protein and ALP significantly declined in fish under low temperature, while a significant increase in AST, ALT, LDH and glucose was observed. The gene expression of antioxidant enzymes (SOD and CAT), HSP90 and C3 were induced by low temperature stress. Furthermore, the gene expression of apoptotic related genes including P53, caspase-9 and caspase-3 were up-regulated, suggesting that caspase-dependent pathway could play important roles in low temperature-induced apoptosis in fish. This study may provide baseline information about how cold stress affects the physiological responses and apoptosis in fish. (C) 2017 Elsevier Ltd. All rights reserved.
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