Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 85, Issue 3, Pages 630-633Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbaa058
Keywords
Heterologous expression; lectin; Pleurocybella porrigens; Escherichia coli
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Funding
- JSPS KAKENHI [JP19K05808, 26850110]
- Novartis Foundation for Gerontological Research
- Sugiyama Chemical & Industrial Laboratory
- UU-COE research project at Utsunomiya University
- Scientific Research on Innovative Areas Frontier Research on Chemical Communications [JP17H06402, 16H02546]
- Grants-in-Aid for Scientific Research [26850110] Funding Source: KAKEN
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This study successfully increased the expression yield of Pleurocybella porrigens lectin using an Escherichia coli-based expression system, resulting in a 9-fold increase compared to previous studies. The recombinant lectin obtained maintained the same biochemical properties as the native lectin.
In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.
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