Green and chemically synthesized zinc oxide nanoparticles: effects on in-vitro seedlings and callus cultures of Silybum marianum and evaluation of their antimicrobial and anticancer potential

Zinc oxide nanoparticles (ZnO-NPs) have been produced by physical and chemical methods. Here, the comparative evaluation of both chemically-synthesised ZnO-NPs (C-ZNPs) and in-vitro cultured S. marianum mediated green-synthesised ZnO-NPs (G-ZNPs) were investigated on seed germination frequency, root and shoot growth, callus induction and biochemical profile of medicinally important plant Silybum marianum. Of all the treatments, callus-mediated ZnO-NPs gave optimum results for seed germination (65%), plantlet's root length (4.3 cm), shoot length (5.3 cm) and fresh and dry weights (220.4 g L-1 and 21.23 g L-1, respectively). Similarly, the accumulation of phenolic (12.3 mu g/mg DW) and flavonoid (2.8 mu g/mg DW) contents were also enhanced in callus cultures treated with G-ZNPs. We also observed maximum antioxidant activity (99%) in callus cultures treated with G-ZNPs, however, in case of plantlets, these activities were found highest for in-vitro whole plant-mediated ZnO-NPs. Moreover, G-ZNPs also enhanced total protein content (265.32 BSAE/20g FW) in callus cultures. G-ZNPs were further assessed for their effects on several multidrug resistant bacterial strains and human liver carcinoma (HepG2) cells and our findings revealed that callus extracts treated with G-ZNPs show ameliorated antibacterial (highest zone of inhibition (19 mm) against Klebsiella pneumonia) and anticancer (highest cytotoxicity of 64%) activities.

Automated summary

The study compared the effects of chemically-synthesised ZnO-NPs and in-vitro cultured S. marianum mediated green-synthesised ZnO-NPs on the growth and biochemical profile of Silybum marianum. The results showed that callus-mediated ZnO-NPs had the most significant positive impact on seed germination, plantlet growth, and biochemical contents. Antioxidant and protein content were also enhanced by G-ZNPs in callus cultures. Additionally, G-ZNPs exhibited improved antibacterial and anticancer activities in multidrug resistant bacterial strains and HepG2 cells.