Journal
CHEMICAL COMMUNICATIONS
Volume 57, Issue 48, Pages 5941-5944Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc01150b
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Funding
- National Natural Science Foundation of China [21804028]
- Key Research and Development Program of Anhui Province [202004h07020029]
- Natural Science Foundation of Anhui Province [1908085QC121]
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This work presents a single-stranded degradable modular grafting probe for analyzing microRNA-21. The exonuclease activity of phi29 polymerase helps reduce background noise, achieving a detection limit of 0.26 fM and showing potential in biochemical analysis.
This work describes a single-stranded degradable modular grafting probe for analyzing microRNA-21. In the system, the exonuclease activity of phi29 polymerase restrains the SYBR Green I/ssDNA induced background. The palindrome activation caused remarkable target fluorescence. The detection limit was achieved as 0.26 fM, showing potential in biochemical analysis.
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