Journal
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
Volume 25, Issue 2, Pages 234-245Publisher
RUSSIAN ACAD SCI, INST CYTOLOGY GENETICS
DOI: 10.18699/VJ21.49-o
Keywords
Klebsiella; molecular serotyping; beta-lactam; fluoroquinolone; aminoglycoside; extended-spectrum beta-lactamases; metallo-beta-lactamases; disco-diffusion analysis; genetic determinants of antibiotic resistance
Funding
- Russian Foundation for Basic Research [18-29-08015]
- [0245-2021-0008]
Ask authors/readers for more resources
A study analyzed 72 clinical strains of Klebsiella spp. isolated from humans in Novosibirsk, Russia and found Klebsiella pneumoniae strains were dominant, with a high percentage of virulent K1- and K2-serotypes. The study also revealed that K. pneumoniae strains isolated from hospitalized patients exhibited higher antibiotic resistance, especially to beta-lactams and carbapenems, and contained genetic determinants for resistance such as bla(CTX) and bla(OXA-48) genes.
72 clinical strains of Klebsiella spp. isolated from samples obtained from humans in Novosibirsk, Russia, were analyzed. Species identification of strains was performed using 16S rRNA and rpoB gene sequences. It was revealed that Klebsiella pneumoniae strains were dominant in the population (57 strains), while the remaining 15 strains were K. grimontii, K. aerogenes, K. oxytoca and K. quasipneumoniae. By molecular serotyping using the wzi gene sequence, K. pneumoniae strains were assigned to twenty-one K-serotypes with a high proportion of virulent K1- and K2-serotypes. It was found that K. pneumoniae strains isolated from the hospitalized patients had a higher resistance to antibiotics compared to the other Klebsiella species. Real-time PCR revealed that the population contained genes of the bla(SHV), bla(TEM), bla(CTX) families and the bla(OXA-48) gene, which are the genetic determinants of beta-lactam resistance. It has been shown that the presence of the bla(CTX) sequence correlated with the production of extended-spectrum beta-lactamases, and phenotypic resistance to carbapenems is due to the presence of the bla(OXA-48) gene. At the same time, the carbapenemase genes vim, ndm, kpc, imp were not detected. Among the aminoglycoside resistance genes studied, the aph(6)-Id and aadA genes were found, but their presence did not always coincide with phenotypic resistance. Resistance to fluoroquinolones in the vast majority of strains was accompanied by the presence of the aac(6')-IB-cr, oqxA, oqxB, qnrB, and qnrS genes in various combinations, while the presence of the oqxA and/or oqxB genes alone did not correlate with resistance to fluoroquinolones. Thus, the detection of bla(CTX) and bla(OXA-48) can be used to quickly predict the production of extended-spectrum beta-lactamases and to determine the resistance of Klebsiella to carbapenems. The detection of the aac(6')-Ib-cr and/or qnrB/qnrS genes can be used to quickly determine resistance to fluoroquinolones.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available