Colorimetric determination of xanthine with xanthine oxidase and WSe2 nanosheets as a peroxidase mimic

A colorimetric method combining WSe2 nanosheets with peroxidase-like activity and xanthine oxidase (XAO) was developed for xanthine detection. XAO catalyzes xanthine oxidation and thereby induces H2O2 production. WSe2 nanosheets can catalyze the oxidization of 3,3 ',5,5 '-tetramethylbenzidine (TMB) by H2O2 and produce a blue oxidation product of TMB (oxTMB). Under optimal conditions, the colorimetric method showed a linear response for xanthine detection in a range of 0.01-0.5 mM. The limit of detection was 4.37 mu M. The recovery rate from spiked serum samples was 95.5%-115.5%. Therefore, our strategy provides new opportunities for the detection of xanthine and evaluation of health care.

Automated summary

A colorimetric method combining WSe2 nanosheets with peroxidase-like activity and xanthine oxidase (XAO) has been developed for xanthine detection. The method shows a linear response for xanthine detection with high recovery rate from spiked serum samples.