Journal
FEBS LETTERS
Volume 591, Issue 2, Pages 393-405Publisher
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12544
Keywords
BC200 RNA; hnRNP E1; hnRNP E2; regulation of translation; RNA-protein interaction
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2011-0020322]
- Intelligent Synthetic Biology Center of Global Frontier Project - MSIP [2013M3A6A8073557]
- National Research Foundation of Korea [2011-0020322] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The long noncoding RNA BC200 ( brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA-inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 30 C-rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA-mediated translation inhibition.
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