Journal
FEBS LETTERS
Volume 591, Issue 6, Pages 833-841Publisher
WILEY
DOI: 10.1002/1873-3468.12597
Keywords
amino-alpha-acetyl-transferase; Nat; Parkinson's disease; Tropomyosin; alpha-synuclein
Funding
- Biotechnology and Biological Sciences Research Council [BB/K012045/1, BB/J012793/1]
- Leverhulme Early Career Fellowship [ECF-2013-341]
- Royal Society Industry Fellowship
- University of Kent
- Biotechnology and Biological Sciences Research Council [1213887, 1405589] Funding Source: researchfish
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Amino-terminal acetylation is a ubiquitous protein modification affecting the majority of eukaryote proteins to regulate stability and function. We describe an optimised recombinant expression system for rapid production of amino terminal-acetylated proteins within bacteria. We go on to describe the system's use in a fluorescence based in vivo assay for use in the high-throughput screen to identify drugs that impact amino-terminal acetylation-dependent oligomerisation. These new tools and protocols will allow researchers to enhance routine recombinant protein production and identify new molecules for use in research and clinical applications.
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