Journal
FEBS LETTERS
Volume 591, Issue 23, Pages 3872-3880Publisher
WILEY
DOI: 10.1002/1873-3468.12893
Keywords
alpha-1-4-glucan protein synthase; protein-protein interactions; reversibly glycosylated polypeptide; Ricinuscommunis (castor oil plant); sucrose synthase; UDP-arabinopyranose mutase
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Queen's Research Chairs program
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The sucrose synthase (SUS) interactome of developing castor oilseeds (COS; Ricinuscommunis) was assessed using coimmunoprecipitation (co-IP) with anti-(COS RcSUS1)-IgG followed by proteomic analysis. A 41-kDa polypeptide (p41) that coimmunoprecipitated with RcSUS1 from COS extracts was identified as reversibly glycosylated polypeptide-1 (RcRGP1) by LC-MS/MS and anti-RcRGP1 immunoblotting. Reciprocal Far-western immunodot blotting corroborated the specific interaction between RcSUS1 and RcRGP1. Co-IP using anti-(COS RcSUS1)-IgG and clarified extracts from other developing seeds as well as cluster (proteoid) roots of white lupin and Harsh Hakea consistently recovered 90 kDa SUS polypeptides along with p41/RGP as a SUS interactor. The results suggest that SUS interacts with RGP in diverse sink tissues to channel UDP-glucose derived from imported sucrose into hemicellulose and/or glycoprotein/glycolipid biosynthesis.
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