Journal
FASEB JOURNAL
Volume 31, Issue 11, Pages 4917-4927Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.201601386R
Keywords
apical sorting; cell polarity; lateral lumens
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Funding
- Taiwan Ministry of Science and Technology (MOST) [103-2320-B-002-049-MY3]
- Taiwan MOST Postdoctoral Fellowship [104-2811-B-002-092, 105-2811-B-002-028]
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Establishment of apical-basal polarity, through correct targeting of polarity determinants to distinct domains of the plasma membrane, is a fundamental process for the development of functioning epithelial tubules. Here we report that galectin (Gal)-8 regulates apical-basal polarity of Madin-Darby canine kidney (MDCK) cells via apical targeting of 135-kDa glycoprotein (Gp135). Gal-8 interacts with newly synthesized Gp135 in a glycandependent manner. Gal-8 knockdown induces aberrant lumens at the lateral domain and mistargeting of Gp135 to this structure, thus disrupting the kidney epithelial polarity of MDCK cells, which organize lumens at the apical surface. The O-glycosylation deletionmutant of Gp135 phenocopies the effect of Gal-8 knockdown, which suggests that Gal-8 is the decoding machinery for the apical sorting signals of Gp135 residing at its O-glycosylation-rich region. Collectively, our results reveal a new role of Gal-8 in the development of luminal organs by regulating targeting of apical polarity protein Gp135.-Lim, H., Yu, C.-Y., Jou, T.-S. Galectin-8 regulates targeting of Gp135/podocalyxin and lumen formation at the apical surface of renal epithelial cells.
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