Potential Chemoprotective Effect of Paederia foetida Linn on Benzyo[a]pyrene induced Gastric Cancer

Benzo[a]pyrene (BaP) exposure is one of the risk factors for lung cancer, but only limited BaP-related data are available on gastrointestinal cancer. This study was aimed at determining protective effects of Paederia foetida L. on benzopyrene (BaP) induced gastric cancer in rats and explore the possible mechanism of action. Paederoside, friedelin, oleanolic acid, sitosterol, linarin, sitogluside, delphinidin, quercetin, peonidin, acacetin, kaempferol, pelargonidin, embelin, and junipene are the main ingredients of P. foetida. Wistar rats (male) were divided into 5 groups: normal control, control supplemented with BaP (20 mg/kg, b.w.), BaP (20 mg/kg, b.w.) group received the P. foetida (25, 50, and 100 mg/kg) for 20 weeks. The experimental protocol was completed and at the end of 20 weeks the rats were sacrificed. Body weight was estimated at regular time intervals. At the end of the study, the hepatic parameters, antioxidants parameters, blood glucose, blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) were estimated. Metabolites of banzopyrene were estimated in the serum. BaP-diolepoxide (BPDE) DNA damage and 8OHdG were estimated at different organs. The expression of cytokines and inflammatory mediators were also estimated. P. foetida treated group rats showed the increased body weight at dose dependent manner. P. foetida significantly (p < 0.001) reduced the hepatic parameters such as AST and ALT. Its also reduced the level of glucose, LDH and increased the level of BUN. P. foetida significantly (p < 0.001) suppressed the level of BaP metabolites such as 3OH BaP, BaP, 7OH BaP and BPDE. It also suppressed the BPDE DNA damage and 8-OhdG in the liver, stomach, and kidney tissue. P. foetida significantly (p < 0.001) improved the level of GPx, SOD, CAT and reduced the level of MDA at dose dependent manner. P. foetida. significantly (p < 0.001) increased the expression of eNOS, TGF-alpha and reduced the expression of IL-1 beta and TNF alpha. Overall, study suggests that the P. foetida treatment considerably inhibited the BaP induced gastric cancer and suggests a promising anticancer effect.

Automated summary

The study demonstrates that Paederia foetida significantly inhibits BaP-induced gastric cancer and shows promising anticancer effects.