A Golgi-localized manganese transporter functions in pollen tube tip growth to control male fertility in Arabidopsis

Manganese (Mn) serves as an essential cofactor for many enzymes in various compartments of a plant cell. Allocation of Mn among various organelles thus plays a central role in Mn homeostasis to support metabolic processes. We report the identification of a Golgi-localized Mn transporter (named PML3) that is essential for rapid cell elongation in young tissues such as emerging leaves and the pollen tube. In particular, the pollen tube defect in the pml3 loss-of-function mutant caused severe reduction in seed yield, a critical agronomic trait. Further analysis suggested that a loss of pectin deposition in the pollen tube might cause the pollen tube to burst and slow its elongation, leading to decreased male fertility. As the Golgi apparatus serves as the major hub for biosynthesis and modification of cell-wall components, PML3 may function in Mn homeostasis of this organelle, thereby controlling metabolic and/or trafficking processes required for pectin deposition in rapidly elongating cells.

Automated summary

A Golgi-localized Mn transporter named PML3 is essential for rapid cell elongation in young tissues like the pollen tube, and its loss-of-function mutation results in reduced seed yield due to decreased male fertility. The lack of pectin deposition in the pollen tube may cause bursting and slow elongation, highlighting the importance of PML3 in Mn homeostasis for metabolic and trafficking processes required for pectin deposition in rapidly elongating cells.