A self-assembled DNA nanostructure as a FRET nanoflare for intracellular ATP imaging

Due to the incorporation of gold nanoparticles (AuNPs), previously reported AuNP-based FRET nanoflares still have some problems, such as non-negligible cytotoxicity and a time-consuming preparation procedure. In this communication, a novel AuNP-free FRET nanoflare for intracellular ATP imaging is developed based on a DNA nanostructure, which is self-assembled through cyclic U-type hybridization only involving a certain number of DNA strands.

Automated summary

In this study, a novel AuNP-free FRET nanoflare for intracellular ATP imaging was developed based on a DNA nanostructure, self-assembled through cyclic U-type hybridization involving a certain number of DNA strands. This new nanoflare addresses the issues of cytotoxicity and time-consuming preparation associated with AuNP-based FRET probes.