Journal
RSC ADVANCES
Volume 11, Issue 27, Pages 16445-16452Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ra02212a
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Funding
- Russian Science Foundation [20-73-00325]
- Russian Science Foundation [20-73-00325] Funding Source: Russian Science Foundation
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Highly sensitive detection of cardiac troponins I and T was achieved through immunochromatography with double amplification using functionalized gold nanoparticles. The robust nature of the approach was confirmed, showing that the choice of optimal assay parameters can enhance sensitivity. The method demonstrated lower limits of detection compared to commonly used systems, with visual limits of detection being 10-fold lower.
Highly sensitive detection of cardiac troponins I and T (cTnI and cTnT) was completed by immunochromatography with double amplification, through the binding of functionalized gold nanoparticles (GNPs). The robust nature of the approach, based on the formation of nanoparticle networks through the biotin-streptavidin interaction, was confirmed; the choice of the best assay parameters for maximal increase in ICA sensitivity was demonstrated. A bifunctional conjugate of GNPs with biotinylated specific IgG and two auxiliary conjugates, GNP-biotin and GNP-streptavidin, form three-component aggregates in the analytical zone of the test strip. The inclusion of abundant gold labels in the resulting immune complex leads to an amplified colorimetric signal. The limits of detection (LoDs) of cTnI and cTnT were 0.9 and 0.4 ng mL(-1), respectively, which is 3 times lower than the LoDs of more commonly used systems. Visual LoDs were 10-fold lower in concentration. The enhancement has been realized both in single and double assay formats; analysis of cTnI and cTnT presented the same characteristics.
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