Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 9, Issue 24, Pages 4906-4914Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0tb02762f
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Funding
- Rosetrees Trust
- German Research Foundation DFG [KL 3191/1-1]
- European Union's Horizon 2020 research and innovation programme under the Marie Skodowska-Curie Actions grant BacDrug [838183]
- ERC Seventh Framework Programme Consolidator Grant 'Naturale CG' [616417]
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- Cancer Research UK [C71717/A30035]
- NanoMed Marie Skodowska-Curie ITN from the H2020 programme [676137]
- European Union's Horizon 2020 research and innovation programme under Marie Skodowska-Curie grant [839111]
- National Institute for Health Research (NIHR) Imperial Biomedical Research Centre (BRC)
- NIAID/NIH [HHSN272200700055C]
- Marie Curie Actions (MSCA) [839111, 838183, 676137] Funding Source: Marie Curie Actions (MSCA)
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The study optimized the engineering of silicon nanowire coated surfaces through chemical etching, and found that increasing interfacing force led to decreased cell viability of Escherichia coli. However, a proportion of the bacterial cell population remained viable despite the aggressive interfacing regime. The silicon nanowires did not result in complete loss of cell viability or partial membrane disruption and corresponding DNA plasmid transformation.
We investigated the biomaterial interface between the bacteria Escherichia coli DH5 alpha and silicon nanowire patterned surfaces. We optimised the engineering of silicon nanowire coated surfaces using metal-assisted chemical etching. Using a combination of focussed ion beam scanning electron microscopy, and cell viability and transformation assays, we found that with increasing interfacing force, cell viability decreases, as a result of increasing cell rupture. However, despite this aggressive interfacing regime, a proportion of the bacterial cell population remains viable. We found that the silicon nanowires neither resulted in complete loss of cell viability nor partial membrane disruption and corresponding DNA plasmid transformation. Critically, assay choice was observed to be important, as a reduction-based metabolic reagent was found to yield false-positive results on the silicon nanowire substrate. We discuss the implications of these results for the future design and assessment of bacteria-nanostructure interfacing experiments.
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