Journal
ANALYST
Volume 146, Issue 13, Pages 4121-4124Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1an00447f
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Funding
- JSPS KAKENHI [JP19J14315, 20H02770]
- Takahashi Industrial and Economic Research Foundation
- Nakatani Foundation
- Izumi Science and Technology Foundation
- Masuya Memorial Basic Research Foundation
- Sumitomo Foundation
- Uehara Memorial Foundation
- Grants-in-Aid for Scientific Research [20H02770] Funding Source: KAKEN
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An enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid was developed for alkaline phosphatase detection, showing higher sensitivity and shorter response time compared to conventional NE.
An enzyme-responsive fluorescent nanoemulsion (NE) based on lipophilic dye liquid (LDL) was developed for alkaline phosphatase (ALP). The response mechanism of the NE involved enzymatic reactions and simultaneous extraction of anions. The LDL-based NE exhibited 3.8 times higher sensitivity than plasticizer-based conventional NE. Detection limit and response range were 2.7 (U L-1) and 5-50 (U L-1), respectively. The response time was reduced to less than half that of the LDL-based membrane.
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