Polymerase-mediated synthesis of p-vinylaniline-coupled fluorescent DNA for the sensing of nucleolin protein-c-myc G-quadruplex interactions

In this paper we report the synthesis of two deoxyuridine derivatives (dU(CN2), dU(Py))-featuring p-vinylaniline-based fluorophores linked through a propargyl unit at the 5' position- that function as molecular rotors. This probing system proved to be useful for the sensing of gene regulation arising from interactions between this G-quadruplex and nucleolin.

Automated summary

This paper reports the synthesis of two deoxyuridine derivatives functioning as molecular rotors, and demonstrates the utility of this probing system in sensing gene regulation arising from interactions between G-quadruplex and nucleolin.