Journal
PLANT COMMUNICATIONS
Volume 2, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xplc.2021.100168
Keywords
CRISPR; RNP; genetic engineering; genome editing; transgene free
Categories
Funding
- National Science Foundation Plant Genome Research Program [IOS-1758745, IOS2029889]
- U.S. Department of Agriculture Biotechnology Risk Assessment Grant Program [2018-33522-28789, 2020-3352232274]
- Emergency Citrus Disease Research and Extension Program [2020-70029-33161]
- Foundation for Food and Agriculture Research grant [593603]
- Syngenta
Ask authors/readers for more resources
CRISPR-derived biotechnologies have revolutionized genetic engineering in plants, but still face challenges such as editing efficiency and off-target mutagenesis.
CRISPR-derived biotechnologies have revolutionized the genetic engineering field and have been widely applied in basic plant research and crop improvement. Commonly used Agrobacterium- or particle bombardment-mediated transformation approaches for the delivery of plasmid-encoded CRISPR reagents can result in the integration of exogenous recombinant DNA and potential off-target mutagenesis. Editing efficiency is also highly dependent on the design of the expression cassette and its genomic insertion site. Genetic engineering using CRISPR ribonucleoproteins (RNPs) has become an attractive approach with many advantages: DNA/transgene-free editing, minimal off-target effects, and reduced toxicity due to the rapid degradation of RNPs and the ability to titrate their dosage while maintaining high editing efficiency. Although RNP-mediated genetic engineering has been demonstrated in many plant species, its editing efficiency remains modest, and its application in many species is limited by difficulties in plant regeneration and selection. In this review, we summarize current developments and challenges in RNP-mediated genetic engineering of plants and provide future research directions to broaden the use of this technology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available