Journal
LAB ON A CHIP
Volume 21, Issue 14, Pages 2730-2737Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1lc00293g
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Funding
- UConn COVID-19 Rapid Start Funding (COVID-RSF) [G401911]
- [R01EB023607]
- [R61AI154642]
- [R01CA214072]
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The study presents an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2, capable of simultaneously detecting nucleoprotein (N) gene, spike (S) gene, and human housekeeping RNAse P gene. This platform shows great potential for rapid, sensitive, reliable, and multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.
The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR-Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 10(2) copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.
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