Journal
STAR PROTOCOLS
Volume 2, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100463
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Funding
- NIH [0255E131, 2T32AG049688-06]
- New York State Department of Health [NYSTEM-C32561GG]
- Michael J. Fox Foundation [0266-3275]
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This study presents a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells, which allows for large-scale production of uniform midbrain organoids for disease modeling and dopaminergic neuron sorting. The long-term maturing organoid cultures can serve as a model for the entire midbrain.
Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for large-scale production of uniform midbrain organoids that can be maintained in diverse formats, and our reporters allow for sorting of dopaminergic neurons. The maturing long-term organoid cultures can be used as a model for the entire midbrain. For complete details on the use and execution of this protocol, please refer to Ahfeldt et al. (2020).
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