Journal
STAR PROTOCOLS
Volume 2, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100485
Keywords
-
Categories
Funding
- Mayo Clinic NIH Grant Relief funding
- NIH [NIH T32 AI07425-23]
Ask authors/readers for more resources
The study presents a flow cytometry protocol for measuring the relative abundance of multiple TFs simultaneously in single cells, enabling direct comparison across different experimental conditions.
Transcription factor (TF) expression levels drive developmental programs, including cell fate and function, and their measurement by flow cytometry allows for robust downstream analysis. However, significant batch-to-batch variability between replicative experiments precludes direct comparison of absolute values across experimental conditions. Here, we present a flow cytometry protocol to measure the relative abundance of multiple TFs simultaneously in single cells, allowing for direct comparison across experimental conditions/time points. This protocol uses bone marrow cells but can be adapted for other cell types. For complete details on the use and execution of this protocol, please refer to Manso et al. (2021) and Manso et al. (2019).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available