Journal
STAR PROTOCOLS
Volume 2, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100527
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [406028471]
- DFG [INST 256/427-1 FUGB]
- Volkswagen Foundation
- [239283807]
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This protocol describes the use of three photoswitchable diacylglycerol analogs for ultra-rapid activation and deactivation of TRPC channels, enabling all-optical stimulation and shutoff in mammalian tissue slices.
Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs-PhoDAG-1, PhoDAG-3, and OptoDArG-for ultrarapid activation and deactivation of native TRPC2 channels in mouse vomeronasal sensory neurons and olfactory type B cells, as well as heterologously expressed human TRPC6 channels. Photoconversion can be achieved in mammalian tissue slices and enables all-optical stimulation and shutoff of TRPC channels.For complete details on the use and execution of this protocol, please refer to Leinders-Zufall et al. (2018).
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