Protocol Protocol for tissue slice cultures from human solid tumors to study therapeutic response

The impact of systemic therapy on the tumor microenvironment has been difficult to study in human solid tumors. Our protocol describes steps for establishing slice cultures to investigate response to chemotherapies, immunotherapies, or adoptive cell therapies. Endpoints include changes in viability, histology, live cell imaging, and multi-omics analyses. The protocol has been applied to a broad array of gastrointestinal malignancies. Culture conditions and treatment parameters can be modified for specific experiments. The platform is highly flexible and easy to manipulate. For complete details on the use and execution of this protocol, please refer to Kenerson et al. (2020), Jabbari et al. (2020), Brempelis et al. (2020), and Jiang et al. (2017).

Automated summary

The protocol outlines steps for establishing slice cultures to investigate the response to chemotherapies, immunotherapies, or adoptive cell therapies in a variety of gastrointestinal malignancies. The culture conditions and treatment parameters can be modified for specific experiments, making the platform highly flexible and easy to manipulate. For more details, please refer to the mentioned studies.