Detection of parallel and antiparallel DNA triplex structures in living human cells using in-cell NMR

We introduced oligodeoxynucleotides (ODNs) that form parallel and antiparallel triplex structures in vitro into living human cells and recorded their in-cell NMR spectra. Observation of landmark signals for triplex structures proved for the first time that parallel and antiparallel triplex structures are formed in living human cells.

Automated summary

Researchers successfully introduced oligodeoxynucleotides that can form triplex structures in vitro into living human cells and observed the characteristic signals of triplex structures through in-cell NMR spectra, providing the first evidence of the formation of parallel and antiparallel triplex structures in living human cells.