Journal
ANALYTICAL METHODS
Volume 13, Issue 27, Pages 3092-3100Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ay00804h
Keywords
-
Funding
- National Key Research and Development Program of China [2019YFC1605004]
Ask authors/readers for more resources
This study developed a highly sensitive monoclonal antibody for colchicine detection, and prepared a lateral-flow immunochromatographic assay strip for on-site detection of colchicine in food samples.
Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. In this study, we developed a highly sensitive monoclonal antibody 1E4 with a half-maximal inhibitory concentration and linear range of 0.43 ng mL(-1) and 0.09-2.16 ng mL(-1), respectively. Using 1E4, we developed a lateral-flow immunochromatographic assay (ICA) strip for COL detection. Based on the results, the detection interval was 1-25 ng mL(-1) in milk, 2.5-50 ng mL(-1) in beef, 1-25 ng mL(-1) in edible lily and 2.5-25 ng mL(-1) in daylily. The lateral-flow ICA strip can be used as an effective tool for COL detection in food samples on site.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available