4.1 Article

Alkaline hydrolysis of olive fruits wastes for the production of single cell protein by Candida lipolytica

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DOI: 10.1016/j.bcab.2021.101999

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Alkali hydrolysis; Candida lipolytica; Contemporary; Oil cake; Olive fruits; SCP

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The study aims to investigate the production of single-cell protein through the hydro-alkaline decomposition of olive residuals using identified yeast Candida lipolytica, with the ideal conditions achieving the highest sugar production and yeast growth.
This study aims to investigate the probable production of single-cell protein (SCP) through an identiied yeast Candida lipolytica 10 DSM 70 56 1, SM -03 01 through the hydro -alkaline decomposition of the olive residuals by the recycling of the olive fruit residues and its use as a carbonic source for yeast growth and to provide the ideal circumstance to gain highest quantity of Carbohydrate of the hydro -alkali product. Alternation of the physical and chemical factors of the nutritional media in order to obtain the highest rate of production. The ideal state of this hydro -decomposition was as follows: 0. 8 M of sodium hydroxide, temperature at 10 0 degrees C and duration time of 60 min. This context provided the highest sugar production, i.e. up to 12 .5 %. The ideal growth of the yeast and production of SCP was after 4 days of incubation with a rotation speed of 15 0 RPM at 30 degrees C and initial pH value of 5. 5 . Moreover, the medium containing 0. 4% peptone as a source was superiors than other used peptone concentration with respect to yeast growth (1 7. 55 g/l) and SCP production (1 1. 24 g/l).

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