Journal
EXPERIMENTAL AND APPLIED ACAROLOGY
Volume 72, Issue 4, Pages 449-459Publisher
SPRINGER
DOI: 10.1007/s10493-017-0167-1
Keywords
Membrane tick feeding; Alternative experimental methods; Hyalomma lusitanicum
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Funding
- Villamagna SA
- [RTA-2014-00080-00-00]
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In vitro feeding of ticks (Acari: Ixodidae) is an important means to study the biology of ticks and their vectorial capacity. Here, we have adapted the tick Hyalomma lusitanicum Koch to previously published silicone-based membranes for in vitro feeding. For comparison purposes data on pre-oviposition, oviposition and hatching from females engorged on animals were used. A total of 68 engorged females out of 169 were obtained; feeding duration and feeding behaviour were similar to that of ticks on live host animals, although the final weight achieved for membrane-fed ticks was lower than that of their animal-fed counterparts. Comparison of the time taken for egg production and hatching showed that pre-oviposition was faster for membrane-fed ticks (16 days) than for animal-fed ticks (36 days), whereas the duration of oviposition-hatching was the same for the two feeding methods (34 days). We also observed that seasonality has an influence on tick feeding success: the conditions in Spring/Summer accelerated the tick life cycle. It is concluded that relatively large numbers of homogeneous laboratory-raised Hyalomma ticks can be produced without feeding them on experimental animals.
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