Journal
RENAL FAILURE
Volume 43, Issue 1, Pages 980-992Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/0886022X.2021.1937219
Keywords
Diabetic glomerulopathy; serum metabolites; mechanism; untargeted metabolomics
Categories
Funding
- National Natural Science Foundation of China [81873611, 81700633]
- Science and Technology Innovation Team of Henan [17IRTSTHN020]
- 2020 Key Project of Medical Science and Technology [SBGJ202002056]
- Foundation for Leading Personnel of Central Plains of China [194200510006]
- National Human Genetic Resources Sharing Service Platform [2005DKA21300]
Ask authors/readers for more resources
The study used untargeted metabolomic techniques to reveal serum metabolite profiles in patients with diabetic glomerulopathy (DG) compared to healthy controls, highlighting the significant role of tryptophan metabolism in the pathogenesis of DG. Differential metabolites were identified, with tryptophan derivatives showing accumulation in DG patients and depletion in healthy controls. Correlation analysis showed associations between specific metabolites and renal function indices in DG patients.
Diabetic glomerulopathy (DG) remains the prevalent microvascular complication and leading cause of shortened lifespan in type-2 diabetes mellitus (T2DM) despite improvement in hyperglycemia control. Considering the pivotal role of kidney in metabolism, using untargeted metabolomic techniques to globally delineate the serum metabolite profiles will help advance understanding pathogenetic underpinnings of renal biopsy-confirmed DG from the perspective of metabolism specifically. Fourteen pathologically diagnosed DG patients secondary to T2DM and 14 age- and gender-matched healthy controls (HCs) were recruited for study. We employed mass spectrometry-based untargeted metabolomic methods to reveal the metabolite profiles of serum samples collected from all included subjects. We identified a total of 334 and 397 metabolites in positive and negative ion mode respectively. One hundred and eighty-two important differential metabolites whose variable importance in projection (VIP) > 1 and p value tryptophan metabolism enriched most significantly. Among the tryptophan derivatives, L-tryptophan (L-Trp) and serotonin were relatively accumulated in DGs compared with HCs, while 5-hydroxyindoleacetic acid (5-HIAA) and indole-3-acetamide were depleted. Correlation analysis showed serotonin and L-Trp are negatively correlated with 24 h urine protein and glycosylated hemoglobin (Ghb). To exclude the interference of preexisting T2DM on DG exacerbation, we selected 5-HIAA and 3-(3-hydroxyphenyl) propionic acid (3-OHPPA) which are not correlated with Ghb and analyzed their correlation relationship with crucial renal indices. We found 3-OHPPA is positively correlated with urine total protein and creatinine ratio (T/Cr) and 24 h urine protein, 5-HIAA is positively correlated with serum creatinine and urea.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available