Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 32, Issue 1, Pages 336-348Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2021.1946486
Keywords
Deoxymiroestrol; fragment antigen-binding (Fab) antibody; enzyme-linked immunosorbent assay (ELISA); Pueraria candollei
Funding
- Graduate School of Khon Kaen University Faculty of Pharmaceutical Sciences, Khon Kaen University [631H115-C]
- International Research Network for Functional Food Discovery & Development (FFDD) of Thailand Science Research and Innovation [IRN61W0005]
- Thailand Research Fund
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A study successfully established an ELISA method for quantifying deoxymiroestrol in Pueraria candollei, demonstrating accurate detection and recovery of the original content. The developed ELISA method showed consistent results with traditional ELISA method, which can be applied for quality control of deoxymiroestrol in Pueraria candollei.
Deoxymiroestrol is the most potent phytoestrogen in the chromene group found in Pueraria candollei. The estrogenic activity of P. candollei, functions as hormone replacement therapy in postmenopausal women. This study established an enzyme-linked immunosorbent assay (ELISA) for quantifying deoxymiroestrol using fragment antigen-binding (Fab) antibody to improve analytical performance. Our ELISA specifically detected deoxymiroestrol in the range of 31.25-1,000 ng/mL. Intra- and inter-assay precision was 1.48%-7.11% and 0.58%-9.31%, respectively, the recovery of authentic deoxymiroestrol spiked into samples was 99.77%-101.61%, and the limit of detection was 30.80 ng/mL. The findings of P. candollei root bark determined using our developed ELISA with Fab antibody and an ELISA with anti-deoxymiroestrol polyclonal antibody were consistent (R-2 = 0.9807). The developed ELISA can be applied to the quality control of deoxymiroestrol derived from P. candollei.
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