Electrospun regenerated Antheraea pernyi silk fibroin scaffolds with improved pore size, mechanical properties and cytocompatibility using mesh collectors

Generally, electrospun silk fibroin scaffolds collected by traditional plates present limited pore size and mechanical properties, which may restrict their biomedical applications. Herein, regenerated Antheraea pernyi silk fibroin (RASF) with excellent inherent cell adhesion property was chosen as a raw material and the conductive metal meshes were used as collectors to prepare modified RASF scaffolds by electrospinning from its aqueous solution. A traditional intact plate was used as a control. The morphology and mechanical properties of the obtained scaffolds were investigated. Schwann cells were further used to assess the cytocompatibility and cell migration ability of the typical scaffolds. Interestingly, compared with the traditional intact plate, the mesh collector with an appropriate gap size (circa 7 mm) could significantly improve the pore size, porosity and mechanical properties of the RASF scaffolds simultaneously. In addition, the scaffold collected under this condition (RASF-7mmG) showed higher cell viability, deeper cell permeation and faster cell migration of Schwann cells. Combined with the excellent inherent properties of ASF and the obviously enhanced scaffold cytocompatibility and mechanical properties, the RASF-7mmG scaffold is expected to be a candidate with great potential for biomedical applications.

Automated summary

By using conductive metal meshes as collectors to prepare modified regenerated Antheraea pernyi silk fibroin (RASF) scaffolds, significant improvements can be made in pore size, porosity, and mechanical properties. The new scaffold (RASF-7mmG) exhibits higher cell viability, deeper cell permeation, and faster cell migration compared to traditional intact plate-collected scaffolds.