4.5 Article

Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016

Journal

EUROSURVEILLANCE
Volume 22, Issue 28, Pages 6-9

Publisher

EUR CENTRE DIS PREVENTION & CONTROL
DOI: 10.2807/1560-7917.ES.2017.22.28.30573

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Funding

  1. Ministry of Environment and Food of Denmark through The Danish Agrifish Agency [33010-NIFA-14612]
  2. Norwegian Veterinary Institute

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We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

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