Cytochrome P450 enzymes in fungal natural product biosynthesis

Covering: 2015 to the end of 2020 Fungal-derived polyketides, non-ribosomal peptides, terpenoids and their hybrids contribute significantly to the chemical space of total natural products. Cytochrome P450 enzymes play essential roles in fungal natural product biosynthesis with their broad substrate scope, great catalytic versatility and high frequency of involvement. Due to the membrane-bound nature, the functional and mechanistic understandings for fungal P450s have been limited for quite a long time. However, recent technical advances, such as the efficient and precise genome editing techniques and the development of several filamentous fungal strains as heterologous P450 expression hosts, have led to remarkable achievements in fungal P450 studies. Here, we provide a comprehensive review to cover the most recent progresses from 2015 to 2020 on catalytic functions and mechanisms, research methodologies and remaining challenges in the fast-growing field of fungal natural product biosynthetic P450s.

Automated summary

Fungal-derived polyketides, non-ribosomal peptides, terpenoids and their hybrids significantly contribute to the chemical space of total natural products from 2015 to end of 2020. Cytochrome P450 enzymes play essential roles in fungal natural product biosynthesis with broad substrate scope and high catalytic versatility. Recent technical advances, such as efficient genome editing techniques and development of filamentous fungal strains as P450 expression hosts, have led to remarkable achievements in fungal P450 studies.