4.5 Article

Coherent anti-stokes Raman scattering spectroscopy (CARS) and imaging of DNA on graphene layers and glass covers

Journal

FLATCHEM
Volume 27, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.flatc.2021.100243

Keywords

Raman scattering; CARS imaging and spectroscopy; DNA molecules; Graphene layer

Funding

  1. HORIZON 2020 project Asymmetry of biological membrane: theoretical, experimental and applied aspects [690853-assymcurv-H2020-MSCA-RISE-2015/H2020MSCA-RISE-2015]
  2. NATO [SPS 985291]
  3. EPSRC [35569/1]
  4. Ukrainian -Lithuanian projects [M/192019]
  5. joint Ukrainian-Czech RD projects [2020.02/0027, 2020.01/0043]

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This study compares the Raman and CARS patterns of DNA molecules deposited on graphene or glass substrates, finding that in DNA CARS spectra, vibrational resonances are red-shifted and exhibit new features in the high-frequency region. The research demonstrates the utility of CARS imaging for visualizing and characterizing DNA molecules deposited on graphene.
We report a comparative study of the Raman and Coherent anti-Stokes Raman scattering (CARS) patterns of DNA molecules deposited on graphene or glass substrates. In the DNA CARS spectra, the positions of vibrational resonances are red-shifted by 10-20 cm(-1) and have new features in high-frequency region in comparison with spontaneous Raman data. We demonstrate that CARS imaging can be employed for the visualization and characterization of DNA molecules deposited on graphene.

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